FIGURE 6.

Mi2β interacts with OGT. a and b, OGT was immunoprecipitated with an OGT-specific antibody (AL-34) in K562 cells before and after NaB induction (a) or hemin and HMBA induction (b), and the blot was probed with Mi2β antibody. c and d, Mi2β protein was immunoprecipitated from K562 cells before and after NaB induction (c) or hemin and HMBA induction (d), and the blot was probed with OGT antibody. The OGA inhibitor TMG was used to increase the overall O-GlcNAc levels. Isotype immunoprecipitation (normal rabbit or mouse IgG) and antibody-alone precipitation (1°) were used as negative controls. All experiments were performed with at least three biological replicates. WB, Western blotting.