FIGURE 7.

Mi2β interacts with OGA. a and b, OGA was immunoprecipitated using an OGA-specific antibody in K562 cells before and after NaB induction (a) or hemin and HMBA induction (b), and the blot was probed with Mi2β antibody. c and d, Mi2β protein was immunoprecipitated from K562 cells before and after NaB induction (c) or hemin and HMBA induction (d), and the blot was probed with OGA antibody. The OGA inhibitor TMG was used to increase overall O-GlcNAc levels. Isotype immunoprecipitation (normal chicken IgY or rabbit IgG) and antibody-alone immunoprecipitation (1°) were used as negative controls. All experiments were performed with at least three biological replicates. WB, Western blotting.