TABLE 3.
Plasmids used in this study
| Plasmid | Notes | Source |
|---|---|---|
| pR413-NEO1 | cen, PNEO1, HIS3 | 34 |
| pR413-neo1-1 | cen, PNEO1, HIS3 | 42 |
| pR413-neo1-2 | cen, PNEO1, HIS3 | 42 |
| pRS416-NEO1 | cen, PNEO1, URA3 | This study |
| pRS416 | cen, URA3 | 51 |
| pRS425 | cen, LEU2 | 55 |
| pRS313 | cen, HIS3 | 55 |
| pRS423 | 2μ, HIS3 | 34 |
| pRS313-DRS2 | cen, PDRS2, HIS3 | This study |
| pRS313-NEO1 | cen, PNEO1, HIS3 | 34 |
| pRS413-ADH-NEO1 | cen, PADH, HIS3 | 34 |
| pRS423-NEO1 | 2μ, PNEO1, HIS3 | This study |
| pRS313-Neo1[D503N] | cen, PNEO1, HIS3 | This study |
| pRS315-DRS2 | cen, PDRS2, LEU2 | 34 |
| pRS425-DRS2 | 2μ, PDRS2, LEU2 | 34 |
| pRS313-DNF1 | cen, PDNF1, HIS3 | 36 |
| pRS423-DNF1 | 2μ, PDNF1, HIS3 | 34, 37 |
| pRS416-DNF1 | cen, PDNF1, URA3 | 33 |
| pRS426-DNF1 | 2μ, PDNF1, URA3 | 33 |
| pRS423-CDC50 | 2μ, PCDC50, HIS3 | 33 |
| pRS425-LEM3 | 2μ, PLEM3, LEU2 | 35 |
| pSKY5/RER1-0 | cen, PTDH3, URA3 | 62 |
| PYM15 | integrating, HIS5, 6XHA | 63 |