FIGURE 7.
Determination of voltage dependence of the slow gate in WT and mutant Cx50 hemichannels. Square voltage pulses were applied to determine voltage dependence. Oocytes maintained in a holding potential of +20 mV were subjected to hyperpolarizing pulses from 20 to −60 mV. Hemichannel currents recorded in oocytes expressing Cx50 (A), Cx50_E42Q (B), and Cx50_F43E (C) mutants. D, graph depicting I(V)/Imax relation of WT Cx50 (black), Cx50_E42Q (green), and Cx50_F43E (brown) mutants. The solid lines represent the fitting of the data to Equation 7 and using the mean values in Table 5. Current traces and corresponding voltage traces have the same color.