TABLE 1.
Site-directed mutagenesis primers
| Mutant | Primer | Sequence |
|---|---|---|
| Cx26_K41N | hCx26_K41N_fw | cctcgttgtggctgcaaatgaggtgtggg |
| hCx26_K41N_rv | cccacacctcatttgcagccacaacgagg | |
| Cx26_E42Q | hCx26_E42Q_fw | catctccccacacctgctttgcagccacaac |
| hCx26_E42Q_rv | gttgtggctgcaaagcaggtgtggggagatg | |
| Cx26_K41N_E42Q | hCx26_K41N_E42Q_fw | cctcgttgtggctgcaaatcaggtgtggg |
| hCx26_K41N_E42Q_rv | cccacacctgatttgcagccacaacgagg | |
| Cx26_K41E | hCx26_K41E_fw | ctcgttgtggctgcagaggaggtgtggg |
| hCx26_K41E_rv | cccacacctcctctgcagccacaacgag | |
| Cx26_K41E_E42K | hCx26_K41E_E42K_fw | gttgtggctgcagagaaggtgtggggagatg |
| hCx26_K41E_E42K_rv | catctccccacaccttctctgcagccacaac | |
| Cx46_E42Q | rCx46_E42Q_Fw | gggcggcagcccaggaggtgtgg |
| rCx46_E42Q_Rv | ccacacctcctgggctgccgccc | |
| Cx46_E43Q | rCx46_E43Q_Fw | cggcagccgagcaggtgtggggtg |
| rCx46_E43Q_Rv | caccccacacctgctcggctgccg | |
| Cx46_E42Q_E43Q | rCx46_E42Q_E43Q_fw | gggcggcagcccagcaggtgtgg |
| rCx46_E42Q_E43Q_rv | ccacacctgctgggctgccgccc | |
| Cx46_E42K | rCx46_E42K_fw | gggcggcagccaaggaggtgtgg |
| rCx46_E42K_rv | ccacacctccttggctgccgccc | |
| Cx46_E43K | rCx46_E43K_Fw | gcggcagccgagaaggtgtggggtg |
| rCx46_E43K_Rv | caccccacaccttctcggctgccgc | |
| Cx50_E42Q | mCx50_E42Q_Fw | cgggacagcagcgcagtttgtgtgggg |
| mCx50_E42Q_Rv | ccccacacaaactgcgctgctgtcccg | |
| Cx50_F43E | mCx50_F43E_Fw | gggacagcagcggaggaggtgtggggcgatgag |
| mCx50_F43E_Rv | ctcatcgccccacacctcctccgctgctgtccc |