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. 2016 Jun 14;291(30):15753–15766. doi: 10.1074/jbc.M116.718023

FIGURE 7.

FIGURE 7.

USP8 depletion decreases APP-C99 and -C89 generation in H4 cells but not APP-CTF degradation. USP8 knockdown decreases the ratio of pC99, C99, pC89, and C89 + pC83 to full-length APP but not C83. A, representative Western blot; B, quantification of full-length APP normalized to GAPDH; *, p = 0.0315. C, ratio of pC99 to APP; *, p = 0.0049. D, ratio of C99 to APP; *, p = 0.0059; E, ratio of pC89 to APP; *, p = 0.0124; F, ratio of C89 + pC83 to APP; *, p = 0.0137; G, ratio of C83 to APP; p = 0.602. USP8 depletion does not affect overexpressed C99 levels in H4-C99 cell line. H, representative Western blot; I, quantification of C99 normalized to GAPDH. Graphs show mean and S.E.; n = 3–4 independent experiments; unpaired t test with Welch's correction, NT, non-targeting.