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o-Tyr was inefficiently acylated to tRNAPhe. Aminoacylation of tRNAPhe by post-transfer editing ablated SccytoPheRS with 75 μm Phe, 75 μm p-Tyr, and 150 μm dl-o-Tyr, expressed as fraction chargeable tRNAPhe ± S.E. (n = 3). tRNAPhe was transcribed in vitro without A76 before addition of A76 with tRNA nucleotidyltransferase using α-[32P]ATP.
