FIGURE 5.
Extent of acetylation of FOXO1, normally and in T2D. The total level of FOXO1 acetylation at Lys262,265,274 (human sequence) was analyzed by SDS-PAGE and immunoblotting in adipocytes from nine non-diabetic control subjects (blue) (mean age, 66 years (range, 27–91); mean BMI, 24 kg/m2 (range, 19–28)) and 10 subjects with type 2 diabetes (red) (mean age, 66 years (range, 28–81); mean BMI, 40 kg/m2 (range, 29–49)). Data are expressed as mean ± S.E. (error bars). a.u., arbitrary units. The non-diabetic control subjects had a mean fasting plasma concentration of glucose of 6.3 mm (range, 4.4–8.0) and a mean concentration of insulin of 53 pm (range, 16–142). Representative immunoblots are shown; each sample represents a single subject with the lower band representing the loading control tubulin (t).