FIGURE 10.
Effect of STAT3 activation by Tyk2 on Cx43 protein level. A, Western blotting analysis of active Tyk2 (p-Tyk2), active STAT3 (p-STAT3), total STAT3, and Cx43 in NRK cells transfected with or without active Tyk2 (Tyk2V678F) and treated with and without SH-4-54 or nifuroxazide. Cx43 protein level was quantified using ImageJ software (*, p < 0.05). B, Western blotting analysis of STAT1, STAT3, and STAT5 from NRK cell lysate. Cell extracts from HeLa cells treated with IFN-α (100 ng/ml) for 5 min were used as a positive control for blotting STAT1 and STAT5. Jurkat cell lysate was used as the positive control for blotting STAT3.