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Effect of Ang II activation of Tyk2 on the phosphorylation level of Cx43. Western blotting analysis of active Tyk2 (p-Tyk2), total Tyk2, pTyr-247, Cx43 pTyr-265, pSer-279/pSer-282, pSer-368, total Cx43, and the angiotensin II type 1 receptor (AT1R) from NRK cells with or without transfected WT Tyk2 and treated with or without Ang II (10−7 m). The protein phosphorylation levels were quantified using ImageJ software (*, p < 0.05; **, p < 0.01).
