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. 2016 May 27;291(30):15867–15880. doi: 10.1074/jbc.M116.727008

FIGURE 2.

FIGURE 2.

Identification of the Cx43CT tyrosine residues phosphorylated by Tyk2. A, sequence of the Cx43CT domain. The Cx43CT tyrosine residues identified from mass spectrometry to be phosphorylated by the Tyk2 catalytic domain in vitro are highlighted (bold and underlined). B, the same in vitro kinase assay as described in A was performed using wild-type Cx43CT236–382 or a Y247,265F (2YF) mutant as substrate, and phosphorylation was detected by Western blotting using a general anti-phosphotyrosine antibody. The control (Ctrl) group did not contain Tyk2. The phosphotyrosine level was quantified using ImageJ software (n = 3, **, p < 0.01).