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Effect of Tyk2 knockdown on the phosphorylation level of Cx43 in LA-25 cells. Western blotting of active Tyk2 (p-Tyk2), total Tyk2, active Src (p-Src), total Src, Cx43 pTyr-247, pTyr-265, pSer-279/pSer-282, pSer-368, and total Cx43 from LA-25 cells at 40 or 35 °C treated with scrambled or Tyk2 siRNA for 12 h. Relative protein levels were quantified using ImageJ software and normalized to the expression level in the scramble RNA-treated sample at 35 °C (*, p < 0.05; **, p < 0.01).
