TABLE 1.
Phosphotyrosine-containing peptides identified from mass spectrometry of the Cx43CT domain phosphorylated in vitro by Tyk2
| Peptide sequence | Start-end positions | Average Mascot Delta Ion Score | No. of phosphopeptidesa | Actual peptide mass | Calculated +1H peptide mass | Phosphorylation site localization |
|---|---|---|---|---|---|---|
| GRSDPyHATTGPLSPSK | 242–258 | 15.35 | 4 | 1849.84 | 1850.84 | Tyr-247 |
| SDPyHATTGPLSPSK | 244–258 | 18.5 | 6 | 1636.72 | 1637.72 | Tyr-247 |
| GRSDPyHATTGPLSPSKDc(carbamidomethyl)GSPK | 242–264 | 9.37 | 2 | 2667.04 | 2668.05 | Tyr-247 |
| QASEQNWANySAEQNR | 304–319 | 13.7 | 2 | 1974.79 | 1975.79 | Tyr-313 |
| yAYFNGc(carbamidomethyl)SSPTAPLSPm(oxidation)SPPGYK | 265–287 | 7.14 | 1 | 2588.07 | 2588.09 | Tyr-265 |
| yAyFNGc(carbamidomethyl)SSPTAPLSPm(oxidation)SPPGYK | 265–287 | 13.8 | 1 | 2667.05 | 2668.05 | Tyr-Y265, Tyr-267 |
a Peptides with a Mascot Delta Ion Score > 7 were used to obtain the average Mascot Delta Ion Score according to Savitski et al. (90).