TABLE 1.

Fitting parameters from multiexponential fitting of TR-FRET between D-FKBP and full-length A-CaM labeled at its N- or C-lobe; effect of S100A1 and Ca²⁺

Data are expressed as means ± S.E. from experimental variation of fits (n = 4 experiments).

RyR	[Ca2+]	[S100A1]	Acceptor at N-lobe site				Acceptor at C-lobe site
			R1	FWHM(R1)	R2	FWHM(R2)	R3	FWHM(R3)
	μm	μm	Å		Å		Å
RyR1	0.03	0	47 ± 0.4	8 ± 0.8	84 ± 4	59 ± 11	96 ± 0.6	35 ± 2
		200	47 ± 0.3	8 ± 0.8	85 ± 4	60 ± 10	96 ± 0.6	34 ± 2
	30	0	48 ± 0.1	18 ± 1.5a	84 ± 2	19 ± 4a	81 ± 0.1a	35 ± 1
		200	48 ± 0.2	11 ± 1.5b	85 ± 3	25 ± 11a	81 ± 0.2a	38 ± 3
RyR2	0.03	0	47 ± 0.6	8 ± 1.0	92 ± 1	27 ± 1	95 ± 2	45 ± 7
		200	48 ± 0.2	7 ± 0.6	95 ± 2	28 ± 8	94 ± 2	35 ± 7
	30	0	47 ± 0.2	8 ± 1.3	79 ± 1a	24 ± 7	81 ± 0.4a	35 ± 4
		200	48 ± 0.2	17 ± 2.7a,b	86 ± 1a,b	30 ± 5	84 ± 1a	49 ± 6

^a Significant effect of Ca²⁺ (p < 0.05), as determined by analysis of variance with Fisher's post hoc test.

^b Significant effect of S100A1 (p < 0.05), as determined by analysis of variance with Fisher's post hoc test.