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. 2016 Jul 22;6:30067. doi: 10.1038/srep30067

Figure 11. Effect of MIF on cell viability and apoptosis of RAW264.7 cells.

Figure 11

RAW264.7 cells were treated with different concentrations of MIF (0, 10, 20, 50, 100, 200 ng/ml) for 24 hrs. In experiment of ISO-1 treatment, RAW264.7 cells were pre-treated with 100 μg/ml ISO-1 for 30 min, following stimulated with 100 ng/ml MIF for 24 hrs. Flow cytometry assay was used to examine the apoptosis of cells and Cell Counting Kit-8 was used to measure cell viability. Bar graphs represent the mean ± SEM of three independent experiments.