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. 2016 Jul 22;6:30177. doi: 10.1038/srep30177

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Copyright © 2016, The Author(s)

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Among the eight Xinjiang wheat cultivars examined, Achun 2, Qichun 5, Tuchun 6 and Yichun 4 carried TaGLDH-A1a whereas Jiuchun 1, Kashibaipi, Redstar and Xinchun 3 had TaGLDH-A1b. (A) Stomatal aperture values measured for control plants (CK) and those treated with PEG 6000 for 24 and 48 h, respectively. The four cultivars with TaGLDH-A1b showed significantly reduced stomatal aperture (filled bars) than those carrying TaGLDH-A1a (open bars) under control conditions and after PEG 6000 treatment for 24 h. Each stomatal aperture value is the mean ± SD of the measurements of at least 200 stomata in six epidermal peels from three plants. The histograms labeled by unidentical letters are statistically different (P < 0.05). The data shown are typical of three independent experiments. (B) Percentages of stomatal aperture decrease after PEG 6000 treatment for 48 h in the eight cultivars. The percentages were calculated using the data depicted in (A). Relative to TaGLDH-A1a lines, the extents of stomatal aperture decrease in those with TaGLDH-A1b (filled bars) were generally and significantly lower.