Figure 2. PCR amplification curves of IAV standards.

The IAV H1N1-derived standards were logarithmically diluted (10⁴ to 10⁰ copies/reaction) for amplification by pan-IAV RT-qPCR. The 10⁴ copies amplification curve crosses the fluorescence threshold at approximately cycle 9, while the 10¹ copy cross at approximately cycle 16. In contrast, the 10⁰ copy does not cross the threshold and is indistinguishable from the negative control.