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. 2016 Jul 22;6:30015. doi: 10.1038/srep30015

Figure 5. Detection of IAV in oral-pharyngeal and cloacal swabs of chickens from a live-bird market by pan-IAV FRET-PCR and virus isolation.

Figure 5

In May of 2016, oral-pharyngeal swabs and cloacal swabs were collected from apparently healthy chickens (n = 60) into 1 ml antibiotics-containing PBS (penicillin 10,000 unit/mL, streptomycin 10 mg/mL, gentamycin 250 μg/mL, kanamycin, 250 μg/mL) for IAV detection by pan-IAV FRET-PCR and virus isolation. The pan-IAV FRET-PCR identified 22 positive chickens (36.7%) with IAV in 17 oral-pharyngeal swabs and 8 cloacal swabs. In contrast, virus isolation determined 17 IAV-positive chickens (28.3%) with 11 positive oral-pharyngeal swabs and 7 positive cloacal swabs. While chickens determined to be positive in cloacal swabs were all positive in oral-pharyngeal swabs by pan-IAV FRET-PCR, one chicken was positive in cloacal swabs but negative in oral-pharyngeal swab by virus isolation. Chickens determined to be positive by virus isolation were all positive based on the pan-IAV FRET-PCR.