Table 1. IAV isolates from Chinese LBMs used for validation and sensitivity determination of PCRs in this study.

IAV strain	IAV subtype	Detection limit
		EID50	Pan-IAV PCR
A/Chicken/Jiangsu/W5-11/2014	H1N1	10−6	10−7
A/Chicken/Jiangsu/Ycd-2/2013	H3N3	10−6	10−7
A/Duck/Jiangsu/W14-5/2014	H4N6	10−7	10−7
A/Duck/Jiangsu/SY/2005	H5N1	10−6	10−7
A/Duck/Jiangsu/W7-5/2013	H6N1	10−4	10−6
A/Chicken/Jiangsu/ZJDT1-8/2014	H7N9	10−8	10−8
A/Chicken/Jiangsu/J10-3/2014	H9N2	10−6	10−6
A//Duck /Jiangsu/J3-5/2014	H10N8	10−6	10−7
A/Duck/Jiangsu/J14-3/2014	H11N2	10−6	10−6