Table 1.
Frequency of protease and reverse transcriptase resistance-related mutations from South African HIV-1 subtype C isolates
| Resistance-associated mutationsa |
Frequency (%) |
Coding nucleotideb |
|
|---|---|---|---|
| Wild type | Mutant | ||
| Protease (n = 29) | |||
| L10V | 2 (7%) | CTT | GTT |
| M36I | 26 (90%) | ATG | ATA/ATC |
| M36L | 3 (10%) | ATG | CTG |
| L60E | 4 (4%) | CTG/CTA/ CTC |
GAA |
| L63S | 3 (10%) | AGC | |
| L63P | 8 (27.5%) | CCC/CCT | |
| L63F | 1 (3%) | TTC | |
| L63T | 1 (3%) | ACT | |
| L63V | 3 (10%) | GTC | |
| A71T | 1 (3%) | GCT | ACT |
| T74S | 2 (6%) | ACC | TCC |
| V77I | 2 (6%) | GTT | ATT |
| V82I | 1 (3%) | GTT | ATT |
| I93L | 27 (94%) | ATT | GTT/CTC |
| Reverse transcriptase (n = 43) | |||
| V90I | 2 (4.6%) | GTT | ATT |
| A98S | 6 (13.9%) | GCC | TCC |
| V90I/K103N | 1 (2.3%) | ||
| K103N | 2 (4.6%) | AAA | AAC |
| V118I | 2 (4.6%) | GCT | ATC |
| E138A | 4 (9.3%) | GAA/GAG | GCA/ GCG |
| V118I/E138A | 1 (2.3%) | ||
| V118I/E138A/ V179D |
1 (2.3%) | ||
| V179I | 3 (6.9%) | GTT | ATC |
a
Resistance mutations were identified according to the Stanford Genotype Resistance Interpretation Algorithm
b
Nonsynonymous codons introducing substitutions at positions related to drug resistance. The wild-type reference is the HIV-1 subtype B consensus sequence