Figure 1. Principle component analysis with Pareto scaling on MS/MS^ALL adipose lipids.

(A-D) Directed principle component analysis (PCA) discrimination of mouse adipose-derived lipid MS/MS^ALL spectra was conducted as described [Ivosev et al., 2008]. PCA of mass spectra data for triacylglycerols (1456 TAGs), diacylglycerols and monoalkyldiacylglycerols (576 DAGs), the SM grouping, including sphingomyelins; cholesteryl esters, ceramides, ceramide phosphates, monohexosylceramides, and dihexosylceramides (541 SMs), and the glycerophospholipid grouping, including cardiolipins, free fatty acids, phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylserine, lysophosphatidylcholine, and lysophosphatidylinositol (1673 GPLs). These data were derived from white adipose (inguinal and perirenal) and interscapular brown adipose depots (n=4 from each depot, analyzed in duplicate). (E, F) Shown are lipids that most differentiated between (E) perirenal and inguinal white adipose and (F) brown adipose and combined white adipose samples (P<0.05).