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. 2016 Jul 22;6:30267. doi: 10.1038/srep30267

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Wt (A,C,E) and Psen1−/− (B,D,F) brain endothelial cells were fixed with acetone/methanol and immunostained for laminin (C,D) and PECAM (CD31; E,F) along with a DAPI nuclear stain (A,B). Panels (G,H) show confocal images of Wt (G) and Psen1−/− (H) endothelial cells immunostained for fibronectin (green) with DAPI counterstaining (blue). Scale bar, 10 μm.