Table 4. Effect of high dosage of purified Cry1C incorporated into the artificial diet on the life-table parameters of P. fuscipes.
| Parameter | Treatments |
||
|---|---|---|---|
| Artificial diet | Artificial diet + 50 μg/g Cry1C | Artificial diet + 1 mg/g PA | |
| 1st instar developmental time (days ± SE)a | 4.4 ± 0.24 (65) | 5.0 ± 0.35 (62) | 3.9 ± 0.76 |
| 2nd instar developmental time (days ± SE)a | 8.4 ± 0.28 (59) | 8.2 ± 0.32 (52) | — |
| Pre-pupa developmental time (days ± SE)a | 1.4 ± 0.07 (55) | 1.3 ± 0.07 (50) | — |
| Pupa developmental time (days ± SE)a | 3.4 ± 0.08 (51) | 3.3 ± 0.07 (49) | — |
| Preimaginal stage duration (days ± SE)a | 17.2 ± 0.31 (51) | 17.4 ± 0.48 (49) | — |
| Preimaginal survival (%)b | 68.0 | 65.3 | 0.027** |
| Female ratio (%)b | 51.0 | 38.8 | — |
| Female body weight (mg ± SE)c | 3.1 ± 0.08 | 2.9 ± 0.07 | — |
| Male body weight (mg ± SE)c | 2.6 ± 0.05 | 2.7 ± 0.07 | — |
The experiment started with 75 larvae per treatment; the experiment lasted until adults eclosed. Larvae of P. fuscipes were fed with an artificial diet containing 50 μg/g Cry1C or 1 mg/g PA (positive control). Pure diet served as a negative control. Number of individuals at each development stage is given in parentheses. Statistical comparisons were made separately for each of the insecticidal compounds compared with the control. Asterisks denote significant differences: P < 0.01.
aMann–Whitney U-test.
bChi-square test.
cStudent’s t-test.