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. 2016 Jul 22;6:30230. doi: 10.1038/srep30230

Figure 2. MRJP1 expressing VSMCs show reduced contractive, migrating and proliferative phenotypes.

Figure 2

(a) Contraction index is significantly reduced in MRJP1 expressing VMSCs compared to control from three independent experiments by collagen gel assay (n = 3, mean ± S.D). Results are shown as mean ± S.D and **p < 0.01. (b) Cell are counted at 24 h, 48 h and 72 h, respectively, to compare the proliferative rates between control and MRJP1 expressing VSMCs (n = 3, mean ± S.D). *p < 0.05, ***p < 0.001. (c) Doubling time (td) of MRJP1 expressing VSMCs is significantly delayed compared to that of controls. td of VSMCs was determined using ln(N48h/N0h) = k(t48h − t0h) and calculating td = ln 2/k when cell number doubling for a given k (n = 3, mean ± S.D). *p < 0.05. (d) Migration index is significantly decreased by the expression of MRJP1 in VSMCs at 6 h and 12 h, respectively by wound healing assay (n = 3, mean ± S.D). *p < 0.05.