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. Author manuscript; available in PMC: 2017 Jul 19.
Published in final edited form as: Immunity. 2016 Jul 19;45(1):83–93. doi: 10.1016/j.immuni.2016.06.022

Figure 2. miR-17-92 deficiency does not impact Foxp3 induction, but enhances Treg cell suppressive function.

Figure 2

(A) In vitro of differentiation of naïve (CD62LhiCD25−CD4+) T cells from WT and mir17-92−/− mice into Th17 and iTreg cell lineages. (B) Analysis of the in vitro function of Treg cells from WT and mir17-92−/− littermates. Treg cells were mixed with CFSE labeled Naïve T lymphocytes from CD45.1+ C57BL/6 mice for three days. CD45.1+ T cells were analyzed for CFSE dilution. (C) The effect of miR-17-92 deficiency on IL-10 expression by Treg cells. mRNA from FACS purified Treg cells was used to generate cDNA for qRT-PCR analysis of IL-10 transcript. Data are representative of 3 independent experiments.