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. 2016 Apr 30;111(3):287–294. doi: 10.1093/cvr/cvw093

Figure 1.

Figure 1

RyR phosphorylation at the CaMKII-specific site is increased in hearts from AnkB+/− mice. (A) Representative immunoblots on heart homogenates from WT and AnkB+/− mice using antibodies that recognize RyR phosphorylated at the CaMKII (S2814) and PKA (S2808) sites and total RyR. (B and C) Ratio of phosphorylated-to-total RyR in hearts from AnkB+/− and WT mice calculated from the relative band intensities. GAPDH was used as loading control. We investigated five hearts/group and immunoblots were repeated four times. Student's t-test was used for statistical analysis. **P < 0.01.