Fig. 3. : mkDNA gene polymerase chain reaction (PCR) products and SL RNA Multiplex PCR resulting from sandfly pools. 2% agarose gel stained with GelRed. (A) mkDNA gene PCR. Lines 1-3: samples from Lutzomyia davisi pools; Line 4: Lu. auraensis/Lu. ruifreitasi pool; Line 5: Leishmania (Leishmania) amazonensis reference strain and Line 6: males of Lutzomyia sp. (B) Multiplex PCR for the Leishmania complex. Line 1: males of Lutzomyia sp.; Lines 2, 5: samples from Lu. davisi pools; Lines 3, 4: samples from Lu. auraensis/Lu. ruifreitasi pools; Lines 6-8 Leishmania spp. reference strains; Line 6: L. (Viannia) braziliensis; 7: L. (L.) amazonensis; 8: L. (L.) infantum chagasi. M: 100 bp size marker (Invitrogen).
