Fig. 5. : mitochondrial H2O2 release and the production of O2- in transfected promastigotes. A: the pTEX or pTEX-heat shock proteins (HSP)70 promastigotes (107 cells/mL) were incubated in PBS/MgCl2 in the presence of 40 μM digitonin, 5 mM succinate, 1 U/mL HRP, 25 μM Amplex Red (Ctrl) and 1.5 μg/mL antimycin A (AA). B: pTEX or pTEX-HSP70 (108 cells/mL) previously loaded with MitoSox were incubated in KH buffer in the presence of 40 μM digitonin, 5 mM succinate (Ctrl) and 1.5 μg/mL antimycin A (AA).
