Figure 3.

Marek's disease virus-chicken host genome interaction phenotypes. The phenotypes shown indicate the interactions and status of the herpesvirus with regard to the chicken host genome, in mitotically dividing cells from the immune organs of MDV-challenged birds (see text for additional descriptions). (a) MDV-null phenotype, lacking MDV FISH signals (FITC, green) in the nucleus or around the chromosomes (DAPI, blue). (b) MDV chromosome-associated phenotype, consisting of diffuse MDV fluorescence signals surrounding the host chromosomes. (c) MDV chromosome-associated/integrated phenotype, defined by the presence of diffuse, host DNA associated MDV signals as well as bright and punctate signals, signifying MDV integrated into host sister chromatids at one or more telomeres. (d) MDV chromosome-integrated only phenotype, consisting of the distinct, punctate MDV FISH signals at the telomeres with no other signals detected.