Table 1.
Protein identification by mass spectrometry (MALDI-TOF MS-MS) after 2-dimensional polyacrylamide gel electrophoresis (2-DE) analysis.
| Spot | Match | Theory | Calculated | Sequence | ||||
|---|---|---|---|---|---|---|---|---|
| no.1 | to2 | Protein name | Score3 | Mw (Da) | pI | Mw (Da) | pI | coverage (%) |
| SERPIN Superfamily | ||||||||
| A14 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 98 | 42,467 | 5.29 | 39,574 | 6.84 | 13 |
| A15 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 170 | 42,467 | 5.29 | 41,542 | 5.11 | 24 |
| A16 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 173 | 41,467 | 5.29 | 34,118 | 5.19 | 20 |
| A21 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 259 | 42,467 | 5.29 | 32,215 | 4.98 | 17 |
| A23 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 174 | 42,467 | 5.29 | 26,100 | 5.07 | 17 |
| A24 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 230 | 42,467 | 5.29 | 23,394 | 5.94 | 14 |
| A25 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 319 | 42,467 | 5.29 | 21,269 | 5.41 | 14 |
| A26 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 233 | 42,467 | 5.29 | 19,760 | 5.14 | 16 |
| A27 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 282 | 42,467 | 5.29 | 19,903 | 5.35 | 18 |
| A28 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 127 | 42,467 | 5.29 | 17,254 | 5.09 | 10 |
| A30 | gi|82204122 | Ovalbumin [Coturnix coturnix] | 182 | 42,467 | 5.29 | 14,448 | 4.74 | 6 |
| A04 | gi|448824824 | ovalbumin-related protein X [Gallus gallus] | 44 | 43,627 | 5.97 | 51,095 | 6.33 | 3 |
| A05 | gi|448824824 | ovalbumin-related protein X [Gallus gallus] | 52 | 43,627 | 5.97 | 50,892 | 6.21 | 10 |
| A06 | gi|448824824 | ovalbumin-related protein X [Gallus gallus] | 44 | 43,627 | 5.97 | 51,915 | 6.06 | 3 |
| A07 | gi|448824824 | ovalbumin-related protein X [Gallus gallus] | 52 | 43,627 | 5.97 | 50,489 | 5.93 | 10 |
| A08 | gi|448824824 | ovalbumin-related protein X [Gallus gallus] | 52 | 43,627 | 5.97 | 50,188 | 5.79 | 10 |
| A09 | gi|448824824 | ovalbumin-related protein X [Gallus gallus] | 44 | 43,627 | 5.97 | 51,095 | 5.65 | 3 |
| A10 | gi|733881415 | ovalbumin-related protein Y [Meleagris gallopavo] | 48 | 43,883 | 5.08 | 45,164 | 5.33 | 11 |
| A11 | gi|733881415 | ovalbumin-related protein Y [Meleagris gallopavo] | 37 | 43,883 | 5.08 | 45,164 | 5.43 | 3 |
| A12 | gi|733881415 | ovalbumin-related protein Y [Meleagris gallopavo] | 37 | 43,883 | 5.08 | 45,074 | 5.56 | 3 |
| A13 | gi|733881415 | ovalbumin-related protein Y [Meleagris gallopavo] | 37 | 43,883 | 5.08 | 45,164 | 5.64 | 3 |
| Transferrin | ||||||||
| A01 | gi|734703936 | transferrin precursor [Meleagris gallopavo] | 254 | 79,552 | 6.62 | 70,764 | 6.61 | 23 |
| BPI Superfamily | ||||||||
| A02 | gi|521300670 | TENP protein [Gallus gallus] | 95 | 49,014 | 5.89 | 48,518 | 6.67 | 11 |
| Lipocalin Superfamily | ||||||||
| A17 | gi|82134837 | Ovoglycoprotein precursor (Alpha 1-acid glycoprotein) [Gallus gallus] | 120 | 22,321 | 5.11 | 31,104 | 5.32 | 15 |
| A29 | gi|13641422 | lipocalin Q83 [Coturnix coturnix] | 210 | 20,368 | 6.75 | 15,777 | 5.85 | 36 |
| Ribosomal_L31e Superfamily | ||||||||
| A18 | gi|115382941 | ribosomal protein L31 [Coturnix coturnix] | 11 | 8,621 | 9.89 | 32,010 | 5.44 | 16 |
| KAZAL_FS Superfamily | ||||||||
| A19 | gi|124763 | Ovomucoid [Coturnix japonica] | 116 | 21,489 | 4.71 | 28,447 | 4.61 | 30 |
| A20 | gi|124763 | Ovomucoid [Coturnix japonica] | 112 | 21,489 | 4.71 | 28,042 | 4.75 | 14 |
| Clusterin | ||||||||
| A22 | gi|520630 | Clusterin [Coturnix coturnix] | 178 | 52,395 | 5.39 | 29,136 | 5.98 | 12 |
| SERPIN Superfamily | ||||||||
| B03 | gi|733881415 | ovalbumin-related protein Y [Meleagris gallopavo] | 48 | 43,883 | 5.08 | 64,884 | 5.16 | 8 |
| B04 | gi|733881415 | ovalbumin-related protein Y [Meleagris gallopavo] | 33 | 43,883 | 5.08 | 55,000 | 5.84 | 9 |
| B06 | gi|874487655 | ovalbumin-related protein X [Anas platyrhynchos] | 83 | 44,185 | 6.29 | 49,583 | 6.78 | 28 |
| B07 | gi|874487655 | ovalbumin-related protein X [Anas platyrhynchos] | 72 | 44,185 | 6.29 | 49,339 | 6.62 | 11 |
| B08 | gi|874487655 | ovalbumin-related protein X [Anas platyrhynchos] | 73 | 44,185 | 6.29 | 48,494 | 6.48 | 20 |
| B09 | gi|874487655 | ovalbumin-related protein X [Anas platyrhynchos] | 75 | 44,185 | 6.29 | 48,614 | 6.33 | 25 |
| B10 | gi|906366229 | Ovalbumin [Anas platyrhynchos] | 84 | 43,396 | 4.96 | 44,261 | 4.58 | 26 |
| B12 | gi|906366229 | Ovalbumin [Anas platyrhynchos] | 216 | 43,595 | 4.96 | 39,884 | 6.92 | 40 |
| B17 | gi|906366229 | Ovalbumin [Anas platyrhynchos] | 61.6 | 43,396 | 4.96 | 23,236 | 5.05 | 14 |
| B18 | gi|906366229 | Ovalbumin [Anas platyrhynchos] | 40.5 | 43,396 | 4.96 | 21,189 | 4.87 | 6 |
| SRCR Superfamily | ||||||||
| B01 | gi|483510368 | Deleted in malignant brain tumors 1 protein [Anas platyrhynchos] | 168 | 54,074 | 4.53 | 153,334 | 5.49 | 20 |
| Transferrin | ||||||||
| B02 | gi|514782616 | Serotransferrin [Anas platyrhynchos] | 411 | 79,064 | 6.40 | 76,083 | 6.72 | 36 |
| Vitellogenin_N | ||||||||
| B05 | gi|483509788 | Vitellogenin-2, partial [Anas platyrhynchos] | 141 | 186,092 | 8.40 | 49,339 | 4.50 | 6 |
| BPI Superfamily | ||||||||
| B11 | gi|541049931 | TENP protein, partial [Anas platyrhynchos] | 60 | 38,566 | 4.55 | 45,704 | 4.87 | 30 |
| Lipocalin Superfamily | ||||||||
| B13 | gi|514739030 | alpha-1-acid glycoprotein-like, partial [Anas platyrhynchos] | 286 | 15,295 | 6.06 | 29,856 | 5.43 | 38 |
| B14 | gi|514739030 | alpha-1-acid glycoprotein-like, partial [Anas platyrhynchos] | 142 | 15,295 | 6.06 | 29,535 | 5.29 | 43 |