In this plot, the solid line represents the 25 kcal/kg formula. The large-dashed line just below the solid line represents the best fit formula for males, 22.7 kcal/kg (95% CI: 20.5, 25.0). The small-dashed line represents the best fit formula for females, 20.6 kcal/kg (95% CI: 19.1, 22.0). Separating males and females allowed detection of bias in the 25 kcal/kg formula, as the 95% CI includes the value 25 for males but not females.