Skip to main content
. 2016 Apr 22;15(13):1706–1714. doi: 10.1080/15384101.2016.1175796

Figure 1.

Figure 1.

Isolation of the U2OS-Phoenix cell line. (a) Cartoon depicting fusion constructs that were introduced into the LoxP site of the AlphoidtetO HAC backbone as markers of CIN within one cell division. N terminal aa 1–99 of hSecurin was fused with tandem copies of enhanced Green Fluorescent Protein (eGFP). Also shown is E. coli tet repressor TetR fused with mCherry (For more details see Materials and Methods). (b) Schematic representation of the HAC containing the constructs with their position and orientation within the HAC (bsr: gene conferring Blasticidin resistance, for more details about HAC construction refer to papers 20 and 21, for more details about loading of the targeting construct into the HAC see Materials and methods). (c) FISH using the FITC-tetO PNA probe detecting the newly constructed HAC in the CHO and U2OS cells. CHO cells containing AlphoidtetO HAC was used as a control. Chromosomes are counterstained with DAPI (blue). Arrows indicate HACs. In the insert, HACs are shown in higher magnification. Size bars = 15 μm.