Table 4.

IC₅₀ values (μg/mL) of free ACGs solution and ACGs-NSps against three tumor cell lines after incubation for 24 hours

Cell line	4T1	HeLa	MCF-7
ACGs-NSps	0.989±0.104*	0.060±0.012**	0.103±0.021**
Free ACGs	1.685±0.357	0.127±0.100	0.273±0.043

Notes: The results are presented as the mean ± SD, n=6.

^*P<0.05 versus ACGs solution,

^**P<0.01 versus ACGs solution.

Abbreviations: ACGs, annonaceous acetogenins; ACGs-NSps, annonaceous acetogenins nanosuspensions; IC₅₀, 50% inhibitory concentration.