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. 2016 Jul 22;12(7):e1006184. doi: 10.1371/journal.pgen.1006184

Fig 5. Ubr1 promotes Guk1-7-GFP degradation.

Fig 5

(A) Wild type and ubr1∆ cells expressing Guk1-7-GFP were incubated with CHX at 25°C or 37°C and samples were analysed by flow cytometry at the indicated time points. The results represent the means and standard deviations of three independent experiments. P values were calculated with an unpaired Student’s t test, *, ** and *** denote P < 0.05, 0.005, and 0.0005, respectively. (B) UBR1 and ubr1∆ cells expressing Guk1-7-GFP along with an empty vector (EV) control or UBR1 were incubated at 37°C and samples were collected at the indicated time points for flow cytometry analysis. Results represent the means and standard deviations of three independent experiments. P values were calculated with a one-way ANOVA and post-hoc Tukey HSD to assess significance, ** denotes P < 0.005. (C) ubr1∆ cells coexpressing Guk1-7-GFP and an empty vector control or either UBR1 or UBR1 (C1220S) were incubated at 37°C with CHX and samples were collected at the indicated time points. (D) Wild type or ubr1∆ cells expressing Guk1 (T290G) fused to GFP were incubated with CHX at 37°C for two hours before being analyzed by flow cytometry. The results represent the relative fluorescence intensities from three independent experiments (with standard deviations). P values were calculated with a one-way ANOVA and post-hoc Tukey HSD to assess significance, *** denotes P < 0.0005.