Fig 3. Enzymatic activity of A.nidulans fucosidase in apoplast from Arabidopsis transgenic plants assayed using 2-Fucosyllactose.

Released fucose was determined by HPAEC-PAD chromatography (as described in Experimental procedures). Means represent average activity determined for three independent transgenic lines and three wild type Col-0 apoplasts. * Differences between transgenic lines and Col-0 are significant (n = 3, p<0.01). (A) Time-course of fucose released from the substrate where 1 mg of total apoplast protein was used. (B) Calculated activity of AnF in Arabidopsis apoplast.