Fig 2. In vivo pollen tube growth is defective in cog3 and cog8 mutants.

(A) to (C) Aniline blue staining of quartets germinated in vivo. (A) In contrast with qrt1^-/- quartets, no more than two normal pollen tubes (arrow) were observed in the mutants. (B) Bright field images of (A). (C) Merged images of (A) and (B). Insets are magnified images of the selected areas in (A) showing that the mutant pollen tubes were arrested at the surfaces of stigma cells (arrowheads). Bars = 20 μm.