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. 2016 Jul 22;16:161. doi: 10.1186/s12866-016-0769-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of exogenous metabolites on susceptibility of A. hydrophila to iron starvation. a–d The growth curve of A. hydrophila strain in 300 μM DIP mM oxaloacetic acid (OAA), α-ketoglutarate (α-KA), succinic acid (SA) and citric acid (CA) treatment, respectively. The concentration of these four reagents are in the gradient of 0, 2, 4 and 8 mM. The growth kinetics of treated cells were recorded by absorbance measurements at OD600 nm, at 30 °C with the Multi-mode detection platform