Fig. 7.

Myeloid-specific ikkβ gene deletion reduces disruption of BBB integrity in the spinal cord during EAE. a-x Spinal cord sections from WT and LysM-Cre/Ikkβ ^F/F EAE mice (n = 5 per group) were double immunostained with anti-PECAM-1 (a-d), fibronectin (m-p), or GFAP (e-h and q-t) antibodies at 15–18 days after immunization. Bars = 10 μm. y-aa Spinal cords from each group (n = 3 per group) were analyzed to test the level of protein expression of PECAM-1 (y and z) and fibronectin (y and aa) with Western blot. Data are represented as mean ± SEM. (ANOVA test; **p < 0.01 versus WT EAE mice; ##p < 0.01 versus normal control mice)