Table 2.
Differentially expressed genes in the parasite life cycle, and primers for qPCR
| Gene IDa | Description | Primersb |
|---|---|---|
| Housekeeping gene | ||
| 18S rRNA | 18S ribosomal RNA (PF3D7_0112300) | AATAACAATGCAAGGCCAATTT CTGCAACAATTTTAATATACGC |
| Young gametocytes | ||
| PFD0310w | Sexual stage-specific protein precursor (Pfs16) | AGTTCTTCAGGTGCCTCTCTTCA AGCTAGCTGAGTTTCTAAAGGCA |
| PF14_0748 | Exported protein (PHISTa), unknown function (Pfg14-748) | CTTATGTGCTGAATTTTGTGTTATGGT TGTAAACTCTGATTTGGCCACACT |
| PF13_0011 | Gamete antigen 27/25 (Pf 27\25) | CATGAAACACATGCCCCTCTCT GCTACAGGCATGAAACTCAATATCC |
| PF14_0744 | Exported protein, unknown function (Pfg14-744) | TTATGTATAATGGCTCTGTTGACGG GGCTTCTCGACTTCCTCGAA |
| PFL0795c | Male development gene 1 (Pfmdv-1/peg3) | TTTGAGACATTTGAACAAGCTTTACA GCATTTCCGGATTTGTTATTTTC |
| PF10_0164 | Early transcribed membrane protein 10.3 (ETRAMP10.3) | TGCTGCTGTTGCTTTGGCTA CCTCAGAGTCGGATCCATCATT |
| Mature gametocytes | ||
| PFF1035w | (Pf77) | GGAAGACAAAAAACACTGCACATTA TTCAAACCATCGTCCTCTTTTTC |
| PFL2405c | Female specific gametocyte-specific (Pfg377) | TGTTCTTTTTCATATCGTCTATCTTCCT TTGCTTTCCTTAAGATGTTTAATGATG |
| PF10_0303 | 25 kDa ookinete surface antigen precursor (Pfs25) | GACTGTAAATAAACCATGTGGAGA CATTTACCGTTACCACAAGTTA |
| PFL2510w | Chitinase (pfcht1) | TCGAGCACGACCAGGTGAA CCTTTCCCACTCTCTTTAAATGTTTT |
| PF10_0302 | 28 kDa ookinete surface protein (Pfs28) | AGAAAATGAAGTGTGTACATTAGAAGGAA GATGTATCAGCCTGGTCCACAGT |
| MAL8.P1.16 | Rhomboid protease ROM3 (ROM3) | AAACTTGAGCACACCAAATGTTCAT CGGTAGCACAAGTCTCCAATATTG |
aGene identification number from PlasmoDB
bPrimers designed using the Primer Express Software (Applied Biosystems). Pfs16 and Pfs25 primers designed by Schneider et al. [21]