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. 2016 Jul 22;16:57. doi: 10.1186/s12935-016-0336-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — CHIR attenuated the inhibitory function of ORI. a and b Cell migration was determined by using wound healing and transwell assay. The migrative ability of CHIR group (2 μM CHIR + 15 μM ORI) was compared with that seen in the ORI group (15 μM). c Real-time PCR analysis of EMT markers were compared between CHIR group(2 μM CHIR + 15 μM ORI) and ORI group (15 μM). d Representative western blots for active β-catenin and β-catenin(total) levels in ORI or CHIR group. Histone H1 and GAPDH were used as internal control. (N, nucleus; C, cytoplasm; W, whole cell). e Immunofluorescence of EMT markers were compared between CHIR group (2 μM CHIR + 15 μM ORI) and ORI group (15 μM). Data are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. ORI group (15 μM)