Fig. 1.

Inhibition of LPS-stimulated platelet-dependent NETosis by rTM. Neutrophils (5 × 10⁴) and platelets (5 × 10⁵) from the same individuals were resuspended in 100 μl of PBS containing 10 % fetal bovine serum. Cultured cells (neutrophils alone or with platelets) were incubated with or without LPS (0.2 μg/ml) in the presence or absence of rTM (2 μg/ml). Immunostaining for MPO and histone H2A.X was performed, and nuclei were stained with DAPI. a–d Normal neutrophils were cultures alone (a), with LPS alone (b), with platelets alone (c), or co-cultured with platelets and LPS (d). e–f rTM was added to the co-culture of neutrophils and platelets with (e) or without LPS (f). Arrows indicate neutrophils that underwent NETosis. Blue, DAPI; green, MPO; red, histone H2A.X; yellow, merged signals. Magnification: ×63, scale bar = 10 μm