Figure 5.

PepT1 expression is up-regulated during colon cancer in mice. (A) The colonic mRNA levels of PepT1 were quantified by quantitative real-time reverse-transcription polymerase chain reaction and normalized to mRNA levels of the ribosomal protein 36B4. Values are means ± SEM (n = 8–9 per group). (B) Immunohistochemical analysis of mouse PepT1 was performed on colonic tissue sections from WT mice treated with AOM (10 mg/kg body weight) plus 2 cycles of 2.5% DSS, or water alone. Microscopic images were taken at 20× magnification. (C) Whole-colon lysates were used in Western blot analyses for mouse PepT1. β-actin was detected as the loading control. **P < .01, ***P < .001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.