Figure 6.

PepT1 expression is up-regulated during colon cancer. (A) Human PepT1 antibody was used to observe PepT1 levels in a human tissue microarray counterstained with hematoxylin. The tissue microarray included samples from normal patients, patients with benign colon tumors, and colon cancer patients with various stages of colon adenocarcinoma. (B) The anti-human PepT1-stained slides were scored according to 2 parameters (from 0 to 2): the intensity of the staining: 0, absence of PepT1-positive cells; 1, low-intensity staining; and 2, high-intensity staining; and the area of positive cells in the epithelium: 0, absence of PepT1-positive cells; 1, less than the half of the epithelium is PepT1 positive; and 2, half or more of the epithelium is PepT1 positive. The intensity was indexed by the surface parameter giving one final score for each slide. Scale bars: 100 μm. *P < .05.