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. Author manuscript; available in PMC: 2016 Jul 22.
Published in final edited form as: Science. 2016 May 26;352(6292):1471–1474. doi: 10.1126/science.aaf7012

Fig. 1. Subclonal lineage tracing of basal epidermal cells.

Fig. 1

(A) Experimental approach for epidermal fate tracking by single-cell label retention. After clonal induction with tamoxifen, K14CreER;R26flox-stop-tTA/mTmG; pTREH2BGFP mice were treated with doxycycline to evaluate H2BGFP label retention. (B) The fate and subsequent behavior of identified cells were determined by live imaging at 2-day intervals. The epidermis is composed of cellular layers starting from the most external, cornified layer, then moving inward to the granular, spinous, and finally basal layer. The dashed outlines indicate the boundaries of the traced clone. (B) and (C) Representative time sequence of a single-cell label retention experiment, showing the resulting lineage tree (C), with individual cell fate choices identified through label dilution within the same clone. Scale bar, 25 μm.