FIG 4.

IC₅₀ determination for hit compounds in the aminoacylation assay compared to IC₅₀s determined in the A/T assay. Titrations of BM03M08 (A), BM04E04 (B), and BM07F03 (C) in the aminoacylation assay were performed. The compound concentrations ranged from 0.4 to 200 μM. Titrations of BM03M08 (D), BM04E04 (E), and BM07F03 (F) in the A/T assay were performed. The concentrations ranged from 5 to 275 μM. Positives were control assays in which there were no compound. The curve fits and IC₅₀s were determined using XLfit 5.3 (IDBS) as part of Microsoft Excel.