TABLE 1.

Primers used for construction and molecular characterization of transgenic P. berghei carrying Pvhppk-dhps

Primer name	Sequence
NdeI_PvHPPK_F	5′-GGGGCATATGGAGGATTCAAACACGGG-3′
BamHI_PvDHPS_+2bp_R	5′-GGGGGGATCCCTAGGTTGATGTATCCTTGTGAG-3′
PvHPPK_V179L_F	5′-AGACCCCCTCGCCATGCTCGTAATTTTAAAGTACATTGAGCA-3′
PvHPPK_V179L_R	5′-TGCTCAATGTACTTTAAAATTACGAGCATGGCGAGGGGGTCT-3′
PvHPPK_M205I_F	5′-GAAATATTTCAAAATCGCATAATAGACATTGACATTTTATTTTTTAAC-3′
PvHPPK_M205I_R	5′-GTTAAAAAATAAAATGTCAATGTCTATTATGCGATTTTGAAATATTTC-3′
PvDHPS_A383G_F	5′-CGGGGGGGAATCGTCCGCCCCTTATGTGGTCCCCAATC-3′
PvDHPS_A383G_R	5′-GATTGGGGACCACATAAGGGGCGGACGATTCCCCCCCG-′3
PvDHPS_A553G_F	5′-GATGTCGGCC TGGGGTTTGCCAAAAAGCACGACCAGTCTATTAAG-3′
PvDHPS_A553G_R	5′-CTTAATAGACTGGTCGTGCTTTTTGGCAAACCCCAGGCCGACATC-3′
ApaI_5′UTR_PbDHPS_F	5′-GGGGGGCCCGTTACACAAATTAGTAGTGTGTC-3′
SalI_3′UTR_PbDHPS_R	5′-GGGGTCGACCAGTTTCTATTAGTTCTTTGATATG-3′
XmaI_3′UTR_PbDHPS_F	5′-GGGCCCGGGCTTCAATGGATAATGTATAGTGG-3′
KasI_3′UTR_PbDHPS_R	5′-GGGGGCGCCG CTATATCTCTCTGTGCTTAC-3′
SalI_Pv_PKDS_F	5′-GGGGTCGACATGGAGGATTCAAACACGGG-3′
SacII_Pv_PKDS_R	5′-GGGCCGCGGCTAAGGTTGATGTATCCTTGTG-3′
PbDHPS_F (a)	5′-ATGGATATTATAGAAGAATCTAATAAATG-3′
3′int_PbDHPS_R (b)	5′-CTGAGACATCAACGTGCCCTC-3′
pL35_before_HindIII_F (c)	5′-GTCTCTTCAATGATTCATAAATAG-3′
PvHPPK_exon1_F (d)	5′-CTCCGTCAGATAGAGCGCCG-3′
pL35_before_Acc65I_F (e)	5′-GACGGTCACAGCTTGTCTGT-3′
pL35_after_KasI_F (f)	5′-CTCATTAGGCACCCCAGGCT-3′
PvDHPS_1767nt_F (g)	5′-CACCCCCGGGGGGAAGGGTGGCGCGGCCATC-3′
Pbshmt_α-tubulin-2_F	5′-GCATGCTGGGAGCTATTTTG-3′
Pbshmt_α-tubulin-2_R	5′-GCTGGTTCAAATGCTGAGTTTG-3′