. 2016 Jul 22;60(8):4453–4463. doi: 10.1128/AAC.01835-15

TABLE 2.

Kinetic parameters of recombinant PvHPPK-DHPS

PvHPPK-DHPS	k_cat (s⁻¹)	K_m pABA (μM)	K_i (μM)
PvHPPK-DHPS	k_cat (s⁻¹)	K_m pABA (μM)	Sulfadoxine	Sulfathiazole	Dapsone
Wild type	0.0024 ± 0.0003	0.15 ± 0.02	1.50 ± 0.11	0.15 ± 0.05	0.11 ± 0.01
A383G mutant	0.0055 ± 0.0005	0.33 ± 0.02	48.10 ± 5.45	2.95 ± 0.11	3.67 ± 0.60
A383G A553G mutant	0.0134 ± 0.0005	0.48 ± 0.05	176.19 ± 8.70	15.53 ± 1.37	4.54 ± 0.51
S382A A383G A553G mutant	0.0060 ± 0.0008	0.26 ± 0.01	266.83 ± 16.31	19.00 ± 1.74	17.73 ± 1.14
V585A mutant	0.0029 ± 0.0002	0.19 ± 0.01	3.01 ± 0.36	0.19 ± 0.02	0.53 ± 0.06