FIG 3.

Effects of pulse duration of different endoperoxides on viability (replication competence) of K13 mutant and revertant parasites. Very-early-ring-stage Cam3.II (top) and Cam3.II_rev (bottom) parasites were incubated with drugs for 3, 6, and 9 h (blue, red, and green symbols, respectively), or the drug pressure was maintained for >48 h (black symbols). For pulse assays, the drugs were removed using the enhanced washing protocols, and parasite viability (rc) was determined after >48 h. Data shown are representative of experimental replicates (n = 2 for pulse assays and n = 3 for >48-h assays). Error bars correspond to the ranges for technical replicates from the individual experiments. Solid curves correspond to the best fits obtained with the CED model, using the parameters for replicate 1 from Table 2. The dashed curves are included as a visual aid to illustrate the dose-response profiles of parasites exposed to drugs for >48 h.