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Loss of MisR increases gonococcal envelope permeability. Gonococci expressing beta-galactosidase were grown in the presence of the membrane-impermeant beta-galactosidase substrate CPRG. Shown are buttons of gonococcal growth after 24 h of standard incubation on supplemented GCB agar containing 100 μg/ml CPRG. Note the increased cleavage of CPRG to release chlorophenol red for misR-deficient strain JK100::PlptA-lacZ. MisR genotypes are noted in parentheses.
