TABLE 5.
Phenotypic characterization of mutations identified during resistance selection
| Antibody used during selection and protein (mutation[s]) | Fold shift in EC50 relative to wild-type BAC-derived HCMVa |
||
|---|---|---|---|
| ARPE-19 | NHDF | HUVEC | |
| LJP538 | |||
| gB (E361K) | 19.1, 26.5 | 1.0, 9.5 | 140.9, 25.1 |
| gB (E381 deletion) | >195.5, >109.2 | >128.2, >149.7 | >284.7, >114.9 |
| gB (D362N) | 0.3, 0.2 | 0.8, 3.2 | 1.8, 2.0 |
| gB (E361K D362N) | 34.3, >109.2 | >128.2, >149.7 | >284.7, >114.9 |
| LJP539, UL130 (Q191K) | >493.7, >405.7 | NAb | >4,714.6, >1,475.1 |
a
EC50 shift was calculated on the basis of the potency of the antibody indicated. Duplicate fold shift values are shown for each mutation. They were obtained from two independent neutralization assays done in triplicate.
b
NA, not applicable.